大鼠TNF alpha ELISA试剂盒(ab108913)
Key features and details
- Sensitivity: 0.0082 ng/ml
- Range: 0.0156 ng/ml - 1 ng/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
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产品名称
大鼠TNF alpha ELISA试剂盒
参阅全部 TNF alpha 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5.9% 批次间 样品 n Mean SD CV% Overall 10.2% -
样品类型
Cell culture supernatant -
检测类型
Sandwich (quantitative) -
灵敏度
= 0.0082 ng/ml -
范围
0.016 ng/ml - 1 ng/ml -
检测时间
4h 30m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Abcam’s TNF alpha Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TNF alpha in rat cell culture supernatants.
A TNF alpha specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TNF alpha X specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TNF alpha captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Rat TNF alpha Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml TNF alpha Microplate (12 x 8 well strips) 1 unit TNF alpha Standard 1 vial -
研究领域
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功能
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
疾病相关
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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别名
- APC1
- APC1 protein
- Cachectin
see all -
数据库链接
- Entrez Gene: 24835 Rat
- SwissProt: P16599 Rat
- Unigene: 2275 Rat
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab108913 被引用在 7 文献中.
- Li J et al. Shugan granule contributes to the improvement of depression-like behaviors in chronic restraint stress-stimulated rats by altering gut microbiota. CNS Neurosci Ther 28:1409-1424 (2022). PubMed: 35713215
- Song P et al. LncRNA MALAT1 Aggravates Renal Tubular Injury via Activating LIN28A and the Nox4/AMPK/mTOR Signaling Axis in Diabetic Nephropathy. Front Endocrinol (Lausanne) 13:895360 (2022). PubMed: 35813614
- Budde H et al. The Interplay between S-Glutathionylation and Phosphorylation of Cardiac Troponin I and Myosin Binding Protein C in End-Stage Human Failing Hearts. Antioxidants (Basel) 10:N/A (2021). PubMed: 34356367
- Kovács Á et al. Interventricular Differences of Signaling Pathways-Mediated Regulation of Cardiomyocyte Function in Response to High Oxidative Stress in the Post-Ischemic Failing Rat Heart. Antioxidants (Basel) 10:N/A (2021). PubMed: 34208541
- Kolijn D et al. Enhanced Cardiomyocyte Function in Hypertensive Rats With Diastolic Dysfunction and Human Heart Failure Patients After Acute Treatment With Soluble Guanylyl Cyclase (sGC) Activator. Front Physiol 11:345 (2020). PubMed: 32523538
- Bao CX et al. GZMB gene silencing confers protection against synovial tissue hyperplasia and articular cartilage tissue injury in rheumatoid arthritis through the MAPK signaling pathway. Biomed Pharmacother 103:346-354 (2018). ELISA . PubMed: 29669300
- Ren T et al. The Combination of Blueberry Juice and Probiotics Ameliorate Non-Alcoholic Steatohepatitis (NASH) by Affecting SREBP-1c/PNPLA-3 Pathway via PPAR-a. Nutrients 9:N/A (2017). ELISA ; Rat . PubMed: 28264426