大鼠IL-4 ELISA试剂盒(without plates) (ab48423)
概述
-
产品名称
大鼠IL-4 ELISA试剂盒(without plates) -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 15 pg/ml -
范围
31.25 pg/ml - 1000 pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Abcam’s IL-4 Rat in vitro ELISA Set is designed for the quantitative measurement of Rat IL-4 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The rat IL-4 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA Set is intended to measure rat IL-4 in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Recognizes both natural and recombinant Rat IL-4 Range: 31.25 pg/ml - 1000 pg/ml Sensitivity < 15 pg/ml
-
经测试应用
适用于: Sandwich ELISAmore details -
平台
Reagents
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 15 x 96 tests 10 x 96 tests 5 x 96 tests Chromogen TMB Substrate Solution 6 vials 4 vials 2 vials Detection biotinylated anti-Rat IL-4 antibody 3 x 0.55ml 2 x 0.55ml 1 x 0.55ml Rat IL-4 Capture antibody 3 x 0.5ml 2 x 0.5ml 1 x 0.5ml Rat IL-4 Standard (Lyophilized) Yellow 15 vials 10 vials 5 vials Streptavidin-HRP 3 vials 2 vials 1 vial -
研究领域
-
相关性
IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL-4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. -
细胞定位
Secreted -
别名
- B cell stimulatory factor 1
- Binetrakin
- BSF1
see all -
数据库链接
- Entrez Gene: 287287 Rat
- SwissProt: P20096 Rat
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab48423于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA |
Use at an assay dependent concentration.
|
说明 |
---|
Sandwich ELISA
Use at an assay dependent concentration. |
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab48423 尚未被引用在任何文献中。