大鼠IL-17A ELISA试剂盒(ab119536)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 1.6 pg/ml - 100 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
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产品名称
大鼠IL-17A ELISA试剂盒
参阅全部 IL-17A 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 7 8.5% 批次间 样品 n Mean SD CV% Overall 7 7.6% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
1 pg/ml -
范围
1.6 pg/ml - 100 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 92% - 112% Serum 34% - 44% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat -
产品概述
Abcam’s IL-17A Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Rat IL-17 (Interleukin-17)concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
IL-17A specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-17A detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-17A captured on the plate.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-rat IL-17A monoclonal antibody 1 x 70µl Microplate coated with monoclonal antibody to rat IL-17A (12 x 8 wells) 1 unit Rat IL-17A Standard Lyophilized (200 pg/mL on reconstitution) 2 vials Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
研究领域
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功能
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
组织特异性
Restricted to activated memory T-cells. -
序列相似性
Belongs to the IL-17 family. -
翻译后修饰
Found both in glycosylated and nonglycosylated forms. -
细胞定位
Secreted. - Information by UniProt
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别名
- CTLA 8
- CTLA-8
- CTLA8
see all -
数据库链接
- Entrez Gene: 301289 Rat
- SwissProt: Q61453 Rat
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab119536 被引用在 4 文献中.
- Yu Y et al. Brain Interleukin-17A contributes to neuroinflammation and cardiac dysfunction in rats with myocardial infarction. Front Neurosci 16:1032434 (2022). PubMed: 36312009
- Zhao Q et al. Chitoheptaose Promotes Heart Rehabilitation in a Rat Myocarditis Model by Improving Antioxidant, Anti-Inflammatory, and Antiapoptotic Properties. Oxid Med Cell Longev 2020:2394704 (2020). PubMed: 32351668
- Liu Z et al. Number 2 Feibi Recipe Reduces PM2.5-Induced Lung Injury in Rats. Evid Based Complement Alternat Med 2018:3674145 (2018). PubMed: 29541141
- Pu J et al. Matrine Exerts a Strong Anti-Arthritic Effect on Type II Collagen-Induced Arthritis in Rats by Inhibiting Inflammatory Responses. Int J Mol Sci 17:N/A (2016). PubMed: 27571073