大鼠ANP ELISA试剂盒(NPPA) (ab108797)
Key features and details
- Sensitivity: 6.1 pg/ml
- Range: 0.008 ng/ml - 2 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
概述
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产品名称
大鼠ANP ELISA试剂盒(NPPA)
参阅全部 Natriuretic peptides A 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 6.3% 批次间 样品 n Mean SD CV% Overall 10.7% -
样品类型
Cell culture supernatant, Serum, Plasma, Tissue -
检测类型
Sandwich (quantitative) -
灵敏度
= 6.1 pg/ml -
范围
0.008 ng/ml - 2 ng/ml -
回收率
102 %
-
检测时间
5h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Rat
预测可用于: Pig -
产品概述
Abcam’s ANP (NPPA) Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of ANP concentrations in cell culture supernatants, tissue-extract, serum and plasma.
An ANP specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an ANP specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of ANP captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 40X Biotinylated Rat ANP Antibody 1 x 150µl ANP Microplate (12 x 8 well strips) 1 unit ANP Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
疾病相关
Atrial standstill 2
Atrial fibrillation, familial, 6 -
序列相似性
Belongs to the natriuretic peptide family. -
翻译后修饰
Cleaved by CORIN upon secretion to produce the functional hormone.
Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity. -
细胞定位
Secreted. - Information by UniProt
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别名
- ANF
- ANF_HUMAN
- ANP
see all -
数据库链接
- Entrez Gene: 397496 Pig
- Entrez Gene: 24602 Rat
- SwissProt: P24259 Pig
- SwissProt: P01161 Rat
图片
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab108797 被引用在 11 文献中.
- Gohar EY et al. Acclimation to a High-Salt Diet Is Sex Dependent. J Am Heart Assoc 11:e020450 (2022). PubMed: 35191321
- Bakrania BA et al. Sustained Elevated Circulating Activin A Impairs Global Longitudinal Strain in Pregnant Rats: A Potential Mechanism for Preeclampsia-Related Cardiac Dysfunction. Cells 11:N/A (2022). PubMed: 35203391
- Ilatovskaya DV et al. Effects of elevation of ANP and its deficiency on cardiorenal function. JCI Insight 7:N/A (2022). PubMed: 35380994
- Chaumais MC et al. Additive protective effects of sacubitril/valsartan and bosentan on vascular remodelling in experimental pulmonary hypertension. Cardiovasc Res 117:1391-1401 (2021). PubMed: 32653925
- Zhang JM et al. BMP-2 alleviates heart failure with type 2 diabetes mellitus and doxorubicin-induced AC16 cell injury by inhibiting NLRP3 inflammasome-mediated pyroptosis. Exp Ther Med 22:897 (2021). PubMed: 34257710