Anti-Raf1抗体[RNP1] (ab50858)
Key features and details
- Mouse monoclonal [RNP1] to Raf1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Raf1抗体[RNP1]
参阅全部 Raf1 一抗 -
描述
小鼠单克隆抗体[RNP1] to Raf1 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Chicken, Xenopus laevis, Orangutan -
免疫原
Synthetic peptide:
GQRDSSYYWEIE
, corresponding to internal sequence amino acids 334-345 of Human Raf1 -
阳性对照
- Extracts and cells from A431 cell line (Human Epidermoid carcinoma)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
RNP1 -
骨髓瘤
NS1 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50858于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 73 kDa.
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ICC/IF |
Use at an assay dependent dilution.
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说明 |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 73 kDa. |
ICC/IF
Use at an assay dependent dilution. |
靶标
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功能
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3. -
组织特异性
In skeletal muscle, isoform 1 is more abundant than isoform 2. -
疾病相关
Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity. -
细胞定位
Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. - Information by UniProt
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数据库链接
- Entrez Gene: 5894 Human
- Entrez Gene: 100173732 Orangutan
- Omim: 164760 Human
- SwissProt: P04049 Human
- SwissProt: Q5R5M7 Orangutan
- SwissProt: P09560 Xenopus laevis
- Unigene: 159130 Human
- Unigene: 5697 Xenopus laevis
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别名
- c Raf antibody
- C-raf antibody
- C-Raf proto-oncogene, serine/threonine kinase antibody
see all
图片
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Lane 1 : Without Raf1 antibody [RNP1] (ab50858)
Lane 2 : Anti-Raf1 antibody [RNP1] (ab50858) at 1 µg/ml
All lanes : A431cell extract (Human Epidermoid carcinoma)
Secondary
All lanes : Goat Anti-Mouse, Peroxidase conjugate
Predicted band size: 73 kDa
Observed band size: 73 kDa -
Localisation of RAF1 protein in the cytoplasm of A431. A431 cells were fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. The cells were incubated with Monoclonal Anti-RAF1 (ab50858) and further developed with Rabbit Anti-Mouse IgG, FITC-conjugate.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab50858 被引用在 6 文献中.
- Wu Q et al. Salvianolic Acid Alleviated Blood-Brain Barrier Permeability in Spontaneously Hypertensive Rats by Inhibiting Apoptosis in Pericytes via P53 and the Ras/Raf/MEK/ERK Pathway. Drug Des Devel Ther 14:1523-1534 (2020). PubMed: 32368011
- Shao Y et al. Circular RNA circDENND2A protects H9c2 cells from oxygen glucose deprivation-induced apoptosis through sponging microRNA-34a. Cell Cycle 19:246-255 (2020). PubMed: 31878833
- Wang H et al. Long non-coding RNA DLX6-AS1 facilitates bladder cancer progression through modulating miR-195-5p/VEGFA signaling pathway. Aging (Albany NY) 12:16021-16034 (2020). PubMed: 32756011
- Zeng M et al. Chinese yam extract and adenosine attenuated LPS-induced cardiac dysfunction by inhibiting RAS and apoptosis via the ER-mediated activation of SHC/Ras/Raf1 pathway. Phytomedicine 61:152857 (2019). PubMed: 31035040
- Jia S et al. Overexpression of indoleamine 2, 3-dioxygenase contributes to the repair of human airway epithelial cells inhibited by dexamethasone via affecting the MAPK/ERK signaling pathway. Exp Ther Med 16:282-290 (2018). PubMed: 29896251
- Chen Q et al. USP10 promotes proliferation and migration and inhibits apoptosis of endometrial stromal cells in endometriosis through activating the Raf-1/MEK/ERK pathway. Am J Physiol Cell Physiol 315:C863-C872 (2018). PubMed: 30281322