Rac2 Activation Assay试剂盒(ab211162)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
概述
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产品名称
Rac2 Activation Assay试剂盒 -
样品类型
Tissue, Adherent cells, Suspension cells -
种属反应性
与反应: Mouse, Human -
产品概述
Rac2 Activation Assay Kit (ab211162) uses PAK1 PBD Agarose beads to selectively isolate and pull-down the active form of Rac from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rac is detected by western blot analysis using an anti-Rac2 specific polyclonal antibody, which reacts with the human and mouse protein.
Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.
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说明
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.
Rac2 (Ras-Related C3 Botulinum Toxin Substrate 2), a 21 kDa protein, belongs to the family of Rho GTPases and regulates a variety of biological response pathways that include secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Like other small GTPases, Rac2 regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active (GTP-bound) state, Rac2 binds specifically to the p21-binding domain (PBD) of p21-activated protein kinase (PAK) to control downstream signaling cascades.
Diseases associated with Rac2 include neutrophil immunodeficiency syndrome and chronic granulomatous disease.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 20 tests ab211177 - PAK1 PBD Agarose Beads 1 x 800µl 100X GTPyS 1 x 50µl 100X GDP 1 x 50µl 5X Assay/Lysis Buffer 1 x 30ml Anti-rac2 Rabbit polyclonal antibody 1 x 40µl Rac2 immunoblot Positive Control 1 x 100µl -
研究领域
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功能
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase. -
组织特异性
Hematopoietic specific. -
疾病相关
Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome (NEUID) [MIM:608203]. -
序列相似性
Belongs to the small GTPase superfamily. Rho family. -
细胞定位
Cytoplasm. Membrane-associated when activated. - Information by UniProt
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别名
- EN 7
- EN-7
- EN7
see all -
数据库链接
- Entrez Gene: 5880 Human
- Entrez Gene: 19354 Mouse
- Omim: 602049 Human
- SwissProt: P15153 Human
- SwissProt: Q05144 Mouse
- Unigene: 517601 Human
- Unigene: 1972 Mouse
相关产品
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图片
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab211162 尚未被引用在任何文献中。