兔抗大鼠IgG H&L (Alkaline Phosphatase) (ab102169)
Key features and details
- Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: IHC-P, ICC, ELISA, WB
概述
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产品名称
兔抗大鼠IgG H&L (Alkaline Phosphatase)
参阅全部 IgG 二抗 -
宿主
Rabbit -
靶标种属
Rat -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. -
经测试应用
适用于: IHC-P, ICC, ELISA, WBmore details -
偶联物
Alkaline Phosphatase
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab102169于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/100 - 1/1000.
|
|
ICC |
1/100 - 1/1000.
|
|
ELISA |
1/1000 - 1/10000. (Primary ELISA).
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|
WB |
1/1000 - 1/10000. (Chemiluminescent).
|
说明 |
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IHC-P
1/100 - 1/1000. |
ICC
1/100 - 1/1000. |
ELISA
1/1000 - 1/10000. (Primary ELISA). |
WB
1/1000 - 1/10000. (Chemiluminescent). |
图片
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All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml (Anti-Tubulin antibody [YL1/2] - Loading Control)
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab102169) at 1/2000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Lanes 3-4 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab102169) at 1/10000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Lanes 5-6 : Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab102169) at 1/20000 dilution (Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP))
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 52 kDa why is the actual band size different from the predicted?
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
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