重组HRP兔IgG,单克隆抗体[EPR25A] -同型对照(ab199507)
Related conjugates and formulations
概述
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产品名称
HRP兔IgG,单克隆抗体[EPR25A] -同型对照
参阅全部 IgG 亚类对照 -
偶联物
HRP -
经测试应用
适用于: IHC-Pmore details -
免疫原
Chemical/ Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods.KLH forms a large complex composed of ~50 kDa subunits.
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常规说明
KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR25A -
同种型
IgG -
研究领域
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细胞定位
Secreted -
别名
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all
相关产品
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Alternative Versions
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
- Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199091)
- Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab199093)
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free) (ab199376)
- Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208150)
- Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208568)
- Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208569)
- PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)
- Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209613)
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
- PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab222107)
- FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab223339)
- APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab199507于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
图片
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IHC image of formalin-fixed paraffin-embedded normal mouse brain sections tested on a Leica BOND™. Sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with antibody (ab185065, Rabbit monoclonal to sodium potassium ATPase, at 1/50 dilution) or isotype control (ab199507, Rabbit IgG, at 1/50 dilution) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX.
The background control image is taken from an identical assay without primary antibody or isotype control.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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IHC image of formalin-fixed paraffin-embedded normal mouse spleen sections tested on a Leica BOND™. Sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with antibody (ab185067, Rabbit monoclonal to alpha tubulin, at 1/100 dilution) or isotype control (ab199507, Rabbit IgG, at 1/50 dilution) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX.
The background control image is taken from an identical assay without primary antibody or isotype control.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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IHC image of formalin-fixed paraffin-embedded normal rat brain sections tested on a Leica BOND™. Sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with antibody (ab185065, Rabbit monoclonal to sodium potassium ATPase, at 1/50 dilution) or isotype control (ab199507, Rabbit IgG, at 1/50 dilution) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX.
The background control image is taken from an identical assay without primary antibody or isotype control.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
IHC image of formalin-fixed paraffin-embedded normal rat spleen sections tested on a Leica BOND™. Sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with antibody (ab185067, Rabbit monoclonal to alpha tubulin, at 1/100 dilution) or isotype control (ab199507, Rabbit IgG, at 1/50 dilution) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX.
The background control image is taken from an identical assay without primary antibody or isotype control.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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IHC image of formalin-fixed paraffin-embedded normal human cerebral cortex sections* tested on a Leica BOND™. Sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with antibody (ab185065, Rabbit monoclonal to sodium potassium ATPase, at 1/50 dilution) or isotype control (ab199507, Rabbit IgG, at 1/50 dilution) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX.
The background control image is taken from an identical assay without primary antibody or isotype control.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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IHC image of formalin-fixed paraffin-embedded normal human spleen sections* tested on a Leica BOND™. Sections were pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The sections were then incubated with antibody (ab185067, Rabbit monoclonal to alpha tubulin, at 1/100 dilution) or isotype control (ab199507, Rabbit IgG, at 1/50 dilution) for 15 mins at room temperature. DAB was used as the chromogen. The sections were then counterstained with haematoxylin and mounted with DPX.
The background control image is taken from an identical assay without primary antibody or isotype control.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
数据表及文件
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SDS download
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Datasheet download
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