Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1 cell line)
Loading amount
10 µg
Specification
THP-1 cell line
Gel Running Conditions
Reduced Denaturing (10% Bis-Tris gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Other product details
Dilution
1/2000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% milk - PBS
Secondary antibody
Name
Non-Abcam antibody was used: Sheep anti-mouse IgG
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 26 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 15 2008