Application
Immunocytochemistry
Sample
Human Cell (human Rab5-transfected HEK cells)
Specification
human Rab5-transfected HEK cells
Fixative
Methanol
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 5%
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
HEK cells were transfected with human Rab5-GFP as described Leterrier et al., 2004. A dilution of ab13253 at 1/200 produced results indistinguishable to those at 1/500. Confocal images revealed good colocalisation of the immunolabelled transfected cells. In confocal image below (left to right): polyclonal rabbit anti-Rab5 (Abcam #13253, 1/500; Cy3; red), and Rab5-GFP (green) with their overlay (far right).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Randal Moldrich
Verified customer
提交于 Dec 02 2005