Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 6% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: trizma saline buffer
Dilution
1/300
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: anti-mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy2 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy2 ®
Additional data
Additional Notes
Immunofluorescent staining for Rab5 using ab66746 (monoclonal from mouse at 1:300) in paraformaldehyde (perfusion) fixed frozen tissue sections from mouse cerebral cortex. Rab5 is revealed with Cy2-conjugated anti-mouse from donkey. Rab5 is apparent in small granules, which are presumed to be endosomes, within the cytoplasm of large pyramidal cells (arrowheads). The Rab5 antibody was successfully combined with other antibodies to reveal cell bodies or receptors of these cells under control and experimental conditions (not shown, Submitted for publication). Scale bar, 10 µm. Thickness, 1,440 nm.
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提交于 Aug 09 2016