Anti-Rab11A抗体(ab65200)
Key features and details
- Rabbit polyclonal to Rab11A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Rab11A抗体
参阅全部 Rab11A 一抗 -
描述
兔多克隆抗体to Rab11A -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Sheep, Chicken, Cow, Rhesus monkey -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Rab11.
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阳性对照
- Recombinant Human Rab11A protein (ab117177) can be used as a positive control in WB. This antibody gave a positive signal in the following Human Whole Cell Lysates: HeLa, Jurkat, MCF7, SHSY-5Y
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65200于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). |
靶标
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功能
Regulates endocytic recycling. May exert its functions by interacting with multiple effector proteins in different complexes. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (By similarity). Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. -
序列相似性
Belongs to the small GTPase superfamily. Rab family. -
细胞定位
Cell membrane. Recycling endosome membrane. Cleavage furrow. Translocates with RAB11FIP2 from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane. Localizes to the cleavage furrow. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis. - Information by UniProt
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数据库链接
- Entrez Gene: 415544 Chicken
- Entrez Gene: 534708 Cow
- Entrez Gene: 8766 Human
- Entrez Gene: 53869 Mouse
- Omim: 605570 Human
- SwissProt: Q5ZJN2 Chicken
- SwissProt: Q2TA29 Cow
- SwissProt: P62491 Human
see all -
别名
- MGC1490 antibody
- Rab 11 antibody
- Rab 11A antibody
see all
图片
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All lanes : Anti-Rab11A antibody (ab65200) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDa
Additional bands at: 26 kDa (possible post-translational modification), 45 kDa, 72 kDa. We are unsure as to the identity of these extra bands.
We believe that the additional bands observed at 45 and 72 kDa may be a result of disulphide-linked forms of the Rab11 protein
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (25)
ab65200 被引用在 25 文献中.
- Scheffler K et al. Two mechanisms drive pronuclear migration in mouse zygotes. Nat Commun 12:841 (2021). PubMed: 33547291
- Actis Dato V & Chiabrando GA Activated Alpha-2 Macroglobulin Improves Insulin Response via LRP1 in Lipid-Loaded HL-1 Cardiomyocytes. Int J Mol Sci 22:N/A (2021). PubMed: 34203120
- Caliva MJ et al. Proteomics analysis identifies PEA-15 as an endosomal phosphoprotein that regulates α5β1 integrin endocytosis. Sci Rep 11:19830 (2021). PubMed: 34615962
- Zhang S et al. Mutant p53 Drives Cancer Metastasis via RCP-Mediated Hsp90a Secretion. Cell Rep 32:107879 (2020). PubMed: 32640214
- Actis Dato V et al. LRP1-Mediated AggLDL Endocytosis Promotes Cholesteryl Ester Accumulation and Impairs Insulin Response in HL-1 Cells. Cells 9:N/A (2020). PubMed: 31936892