Application
Western blot
Sample
Mouse Cell lysate - nuclear (ES cells)
Loading amount
100000 cells
Specification
ES cells
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 32 kDa Non-specific: 24 kDa
Positive control
Hela nuclear extract
Additional data
Additional Notes
Primary incubated in TBS pH8.0, Secondary incubated in TBS pH 8.0 5% milk. Washes done on TBS pH 8.0
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Ligia Tavares
Verified customer
提交于 Mar 18 2011