Application
Western blot
Sample
Human Cell lysate - whole cell (macrophags)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
1e+006 cells
Specification
macrophags
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/900
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 650
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 650
Detection
Detection method
odssey
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 52 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 09 2014