重组Anti-RUNX1 / AML1 + RUNX3 + RUNX2抗体[EPR3099] (ab92336)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3099] to RUNX1 / AML1 + RUNX3 + RUNX2
- Suitable for: ChIC/CUT&RUN-seq, Flow Cyt (Intra), WB, IP, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-RUNX1 / AML1 + RUNX3 + RUNX2抗体[EPR3099]
参阅全部 RUNX1 / AML1+RUNX3+RUNX2 一抗 -
描述
兔单克隆抗体[EPR3099] to RUNX1 / AML1 + RUNX3 + RUNX2 -
宿主
Rabbit -
经测试应用
适用于: ChIC/CUT&RUN-seq, Flow Cyt (Intra), WB, IP, IHC-P, IHC-Frmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab177141) -
阳性对照
- WB: MOLT4, WEHI-3, CTLL-2 and Raw264.7 cell lysate; mouse and rat thymus tissue lysate, mouse spleen tissue lysate and fetal thymus tissue lysate. IHC: Human tonsil tissue. IP: Molt-4 cell lysate IHC-Fr: Human tonsil tissue sections. ChIC/CUT&RUN-Seq: K-562 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR3099 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 488 Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab199221)
- Alexa Fluor® 647 Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab199493)
- Alexa Fluor® 594 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099] (ab207251)
- Alexa Fluor® 555 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099] (ab207253)
- Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free (ab220117)
- Alexa Fluor® 568 Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab312598)
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92336于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ChIC/CUT&RUN-seq |
Use at an assay dependent concentration.
5 µg |
|
Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/5000 - 1/10000. Predicted molecular weight: 49 kDa.Can be blocked with RUNX1 / AML1 peptide (ab177141).
|
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IP |
1/20.
|
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
The use of an HRP/AP polymerized secondary antibody will give a stronger signal. |
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IHC-Fr |
1/500.
|
说明 |
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ChIC/CUT&RUN-seq
Use at an assay dependent concentration. 5 µg |
Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/5000 - 1/10000. Predicted molecular weight: 49 kDa.Can be blocked with RUNX1 / AML1 peptide (ab177141). |
IP
1/20. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody will give a stronger signal. |
IHC-Fr
1/500. |
靶标
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细胞定位
RUNX1 / AML1: Nucleus. RUNX3: Nucleus. Cytoplasm. The tyrosine phosphorylated form localizes to the cytoplasm. RUNX2: Nucleus. -
数据库链接
- Entrez Gene: 860 Human
- Entrez Gene: 861 Human
- Entrez Gene: 864 Human
- Entrez Gene: 12393 Mouse
- Entrez Gene: 12394 Mouse
- Entrez Gene: 12399 Mouse
- Entrez Gene: 156726 Rat
- Entrez Gene: 367218 Rat
see all
图片
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ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 K-562 (Human chronic myelogenous leukemia lymphoblast) cells and 5µg of ab92336 [EPR3099]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.
Additional screenshots of mapped reads can be downloaded here.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
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All lanes : Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336) at 1.28 µg/ml (purified)
Lane 1 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : Molt-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lane 3 : WEHI-3 (Mouse leukemia lymphoblast) whole cell lysate
Lane 4 : Mouse thymus lysate
Lane 5 : CTLL-2 (Mouse T lymphocyte) whole cell lysate
Lane 6 : Rat thymus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 49 kDaBlocking/Diluting buffer and concentration: 5% NFDM /TBST
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ab92336 (purified) at 1/20 immunoprecipitating RUNX1 / AML1 + RUNX3 + RUNX2 in 10 μg Molt-4 (Human lymphoblastic leukemia T lymphoblast)whole cell lysate (Lanes 1 and 2, observed at 49 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730) instead of ab92336 in Molt-4 whole cell lysate. For western blotting, ab92336 at 1/500 and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336) at 1.28 µg/ml (purified)
Lane 1 : Mouse spleen lysate
Lane 2 : Mouse thymus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 49 kDaBlocking/Diluting buffer and concentration: 5% NFDM /TBST
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RUNX2 recombinant protein full length, with N-terminal HIS tag, expressed in E.Coli.
RUNX3 overexpression and empty vector control lysates created in HEK293T cells. The protein contains a C-terminal DDK tag.
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All lanes : Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336) at 1/10000 dilution
Lane 1 : MOLT4
cell lysate
Lane 2 : fetal thymus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 49 kDa -
IHC image of RUNX1 / AML1 + RUNX3 + RUNX2 staining in a section of frozen normal human tonsil performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab92336, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry staining of RUNX1 / AML1 in formalin-fixed, paraffin-embedded Human tonsil tissue using 1/100 ab92336. Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of permeabilized Molt-4 cells using anti-RUNX1 ab92336 (red) or a rabbit IgG (negative) (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (75)
ab92336 被引用在 75 文献中.
- Peng S et al. LncRNA-AK137033 inhibits the osteogenic potential of adipose-derived stem cells in diabetic osteoporosis by regulating Wnt signaling pathway via DNA methylation. Cell Prolif 55:e13174 (2022). PubMed: 34953002
- He X et al. FNDC5/irisin facilitates muscle-adipose-bone connectivity through ubiquitination-dependent activation of runt-related transcriptional factors RUNX1/2. J Biol Chem 298:101679 (2022). PubMed: 35124008
- Ma L et al. Small extracellular vesicles from dental follicle stem cells provide biochemical cues for periodontal tissue regeneration. Stem Cell Res Ther 13:92 (2022). PubMed: 35241181
- Porras DP et al. Challenges in Cell Fate Acquisition to Scid-Repopulating Activity from Hemogenic Endothelium of hiPSCs Derived from AML Patients Using Forced Transcription Factor Expression. Cells 11:N/A (2022). PubMed: 35741044
- Sá da Bandeira D et al. PDGFRβ+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny. Cell Rep 40:111114 (2022). PubMed: 35858557