重组Anti-RUNX1 / AML1 + RUNX3 + RUNX2抗体[EPR3099] - BSA and Azide free (ab220117)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3099] to RUNX1 / AML1 + RUNX3 + RUNX2 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), ChIC/CUT&RUN-seq, IHC-Fr, WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-RUNX1 / AML1 + RUNX3 + RUNX2抗体[EPR3099] - BSA and Azide free -
描述
兔单克隆抗体[EPR3099] to RUNX1 / AML1 + RUNX3 + RUNX2 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ChIC/CUT&RUN-seq, IHC-Fr, WB, IHC-P, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab177141) -
阳性对照
- WB: MOLT4, WEHI-3, CTLL-2 and Raw264.7 cell lysate; mouse and rat thymus tissue lysate, mouse spleen tissue lysate and fetal thymus tissue lysate. IHC: Human tonsil tissue. IP: Molt-4 cell lysate IHC-Fr: Human tonsil tissue sections. ChIC/CUT&RUN-Seq: K-562 cells.
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常规说明
ab220117 is the carrier-free version of ab92336.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3099 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 488 Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab199221)
- Alexa Fluor® 647 Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab199493)
- Alexa Fluor® 594 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099] (ab207251)
- Alexa Fluor® 555 Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3099] (ab207253)
- PE Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab305695)
- APC Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab305696)
- HRP Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab305697)
- Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336)
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab220117于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ChIC/CUT&RUN-seq |
Use at an assay dependent concentration.
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IHC-Fr |
1/500.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.Can be blocked with RUNX1 / AML1 peptide (ab177141).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
The use of an HRP/AP polymerized secondary antibody will give a stronger signal. |
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IP |
1/50.
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说明 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ChIC/CUT&RUN-seq
Use at an assay dependent concentration. |
IHC-Fr
1/500. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.Can be blocked with RUNX1 / AML1 peptide (ab177141). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody will give a stronger signal. |
IP
1/50. |
靶标
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细胞定位
RUNX1 / AML1: Nucleus. RUNX3: Nucleus. Cytoplasm. The tyrosine phosphorylated form localizes to the cytoplasm. RUNX2: Nucleus. -
数据库链接
- Entrez Gene: 860 Human
- Entrez Gene: 861 Human
- Entrez Gene: 864 Human
- Entrez Gene: 12393 Mouse
- Entrez Gene: 12394 Mouse
- Entrez Gene: 12399 Mouse
- Entrez Gene: 156726 Rat
- Entrez Gene: 367218 Rat
see all
图片
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ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2.5 x 10^5 K-562 (Human chronic myelogenous leukemia lymphoblast) cells and 5µg of ab92336 [EPR3099]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.
Additional screenshots of mapped reads can be downloaded here.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92336).
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All lanes : Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336) at 1.28 µg/ml (purified)
Lane 1 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : Molt-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lane 3 : WEHI-3 (Mouse leukemia lymphoblast) whole cell lysate
Lane 4 : Mouse thymus lysate
Lane 5 : CTLL-2 (Mouse T lymphocyte) whole cell lysate
Lane 6 : Rat thymus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 49 kDaBlocking and diluting buffer: 5% NFDM/TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92336).
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All lanes : Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] (ab92336) at 1.28 µg/ml (purified)
Lane 1 : Mouse spleen lysate
Lane 2 : Mouse thymus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 49 kDaBlocking/Diluting buffer and concentration: 5% NFDM /TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92336).
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ab92336 (purified) at 1/50 immunoprecipitating RUNX1 / AML1 + RUNX3 + RUNX2 in 10 μg Molt-4 (Human lymphoblastic leukemia T lymphoblast)whole cell lysate (Lanes 1 and 2, observed at 49 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730) instead of ab92336 in Molt-4 whole cell lysate. For western blotting, ab220117 at 1/500 and HRP Veriblot for IP (ab131366) was used for detection at 1/1000 dilution.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92336).
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This data was developed using the same antibody clone in a different buffer formulation (ab92336).
IHC image of RUNX1 / AML1 + RUNX3 + RUNX2 staining in a section of frozen normal human tonsil performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab92336, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry staining of RUNX1 / AML1 in formalin-fixed, paraffin-embedded Human tonsil tissue using 1/100 ab92336.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92336).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab92336 staining RUNX1 / AML1 in human glioblastoma cell line by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab92336 at a 1/50 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/400 dilution. Nuclei are counterstained with DAPI.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92336).
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ab92336 staining RUNX1 / AML1 in rat glioblastoma cell line C6 by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab92336 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a Cy3 conjugated goat anti-rabbit polyclonal used at a 1/400 dilution. Nuclei are counterstained with DAPI.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92336).
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Lane 1 (input): MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate, 10μg
Lane 2 (+): MOLT-4 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab220117 in MOLT-4 whole cell lysateAb220117 Immunoprecipitating RUNX1 / AML1 + RUNX3 + RUNX2 in MOLT-4 whole cell lysates. For western blotting, primary antibody used was ab220117 at 1:500 dilution (1.98 µg/ml). Ab131366 VeriBlot for IP (HRP) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
实验方案
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (9)
ab220117 被引用在 9 文献中.
- Ponder KL et al. Preeclampsia and Inflammatory Preterm Labor Alter the Human Placental Hematopoietic Niche. Reprod Sci 23:1179-92 (2016). PubMed: 26944948
- Treanor LM et al. Interleukin-7 receptor mutants initiate early T cell precursor leukemia in murine thymocyte progenitors with multipotent potential. J Exp Med 211:701-13 (2014). PubMed: 24687960
- Vacchio MS et al. A ThPOK-LRF transcriptional node maintains the integrity and effector potential of post-thymic CD4(+) T cells. Nat Immunol 15:947-56 (2014). PubMed: 25129370
- Padrón-Barthe L et al. Clonal analysis identifies hemogenic endothelium as the source of the blood-endothelial common lineage in the mouse embryo. Blood 124:2523-32 (2014). PubMed: 25139355
- García-González E & Recillas-Targa F A regulatory element affects the activity and chromatin structure of the chicken a-globin 3' enhancer. Biochim Biophys Acta 1839:1233-41 (2014). PubMed: 25239823
- Ding G et al. PDGF receptor alpha+ mesoderm contributes to endothelial and hematopoietic cells in mice. Dev Dyn 242:254-68 (2013). ICC/IF ; Mouse . PubMed: 23335233
- Tober J et al. Distinct temporal requirements for Runx1 in hematopoietic progenitors and stem cells. Development 140:3765-76 (2013). Mouse . PubMed: 23924635
- Chiang PM & Wong PC Differentiation of an embryonic stem cell to hemogenic endothelium by defined factors: essential role of bone morphogenetic protein 4. Development 138:2833-43 (2011). ICC/IF . PubMed: 21613326
- Ficarro SB et al. Online nanoflow multi-dimensional fractionation for high efficiency phosphopeptide analysis. Mol Cell Proteomics : (2011). WB ; Mouse . PubMed: 21788404