Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Specification
Colon
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Other product details
Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA buffer, pH 7.3
Secondary antibody
Name
Non-Abcam antibody was used: Rabbit anti-mouse
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/200
Additional data
Additional Notes
No antigen retrieval is required for this antibody. Adding heat-mediated antigen retrieval in citric buffer, pH6 did not alter staining patter. Primary antibody was incubated for over-night at 4C and this produced superior staining results comparing to shorter primary antibody incubation at room temperature.
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提交于 May 26 2011