Application
Western blot
Sample
Human Cell lysate - whole cell (A549 tumour cell line)
Loading amount
50 µg
Specification
A549 tumour cell line
Treatment
1h with DNA-PK inhibitor +/- 10IU Bleomycin (4h)
Gel Running Conditions
Reduced Denaturing (10% Tris Glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in TNT
Secondary antibody
Name
Non-Abcam antibody was used: Bio-Rad goat antiRabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 32 kDa Non-specific: 0 kDa
Additional data
Additional Notes
we used 5% milk as a blocking agent as it gave a better result than using BSA
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 11 2010