Application
Western blot
Sample
Human Cell lysate - whole cell (Breast Cancer Cell Line)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Treatment
3mM Hydroxyurea 5hrs
Specification
Breast Cancer Cell Line
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA + Phosphatase Inhibitors
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: anit-Rabbit HRP
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 35 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 06 2016