Anti-RPA32/RPA2抗体(ab97594)
Key features and details
- Rabbit polyclonal to RPA32/RPA2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-RPA32/RPA2抗体
参阅全部 RPA32/RPA2 一抗 -
描述
兔多克隆抗体to RPA32/RPA2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 172 of human RPA32/RPA2 (NP_002937)
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阳性对照
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates A549 xenograft HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97594于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 29 kDa.
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IHC-P |
1/100 - 1/250.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 29 kDa. |
IHC-P
1/100 - 1/250. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. -
翻译后修饰
Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1. -
细胞定位
Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage. - Information by UniProt
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数据库链接
- Entrez Gene: 507912 Cow
- Entrez Gene: 6118 Human
- Entrez Gene: 19891 Mouse
- Entrez Gene: 59102 Rat
- Omim: 179836 Human
- SwissProt: P15927 Human
- SwissProt: Q62193 Mouse
- SwissProt: Q63528 Rat
see all -
别名
- 60S acidic ribosomal protein P1 antibody
- AA409079 antibody
- AI325195 antibody
see all
图片
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All lanes : Anti-RPA32/RPA2 antibody (ab97594) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
Gel conc 12% (SDS PAGE) -
ab97594, at a 1/100 dilution, staining human RPA32/RPA2 in A549 xenograft, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97594 at a 1/200 dilution (top image) and merged with DNA probe (bottom image).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab97594 被引用在 2 文献中.
- Izumi H & Funa K Telomere Function and the G-Quadruplex Formation are Regulated by hnRNP U. Cells 8:N/A (2019). PubMed: 31035352
- Chan YW et al. Unresolved recombination intermediates lead to ultra-fine anaphase bridges, chromosome breaks and aberrations. Nat Cell Biol 20:92-103 (2018). PubMed: 29255170