Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Liver)
Specification
Liver
Fixative
Methanol
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Other product details
Dilution
1/100
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+0.3% Triton-X
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
I tried it on rat liver but it did not work
Abcam response
Thank you for your valuable feedback. We are sorry to hear that this antibody has not given you good results in IHC frozen section using rat sample. This antibody has not been tested with this species before, and it is possible that it may not be suitable for use.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Aug 06 2012