Anti-ROR alpha/RORA抗体(ab70061)
Key features and details
- Rabbit polyclonal to ROR alpha/RORA
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
概述
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产品名称
Anti-ROR alpha/RORA抗体
参阅全部 ROR alpha/RORA 一抗 -
描述
兔多克隆抗体to ROR alpha/RORA -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human, African green monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human breast carcinoma tissue, extracts from HeLa and COS-7 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70061于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
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IHC-P |
1/50 - 1/100.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
IHC-P
1/50 - 1/100. |
靶标
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功能
Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives. -
组织特异性
Widely expressed in a number of tissues. -
序列相似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
翻译后修饰
Phosphorylation by PKC in neurons inhibits transcriptional activity. Phosphorylated on Thr-216 by ERK2 in vitro.
Sumoylated by SENP1 and SENP2. Sumoylation, promoted by PIAS2, PIAS3, PIASy but not PIAS1, enhances the transcriptional activity. Desumoylated by SENP1.
Ubiquitinated. Ubiquitination is required for efficient transcriptional activity and is prevented by Hairless. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6095 Human
- Omim: 600825 Human
- SwissProt: P35398 Human
- Unigene: 560343 Human
- Unigene: 655155 Human
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别名
- fhl2a antibody
- NR1F1 antibody
- Nuclear receptor ROR alpha antibody
see all
图片
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All lanes : Anti-ROR alpha/RORA antibody (ab70061) at 1/500 dilution
Lane 1 : extract from HeLa cells
Lane 2 : extract from COS-7 cells
Lane 3 : extract from COS-7 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 63 kDa
Observed band size: 63 kDa -
Human breast carcinoma tissue with ab70061 at 1/50 dilution, with and without the immunising peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab70061 被引用在 1 文献中.
- Kang X et al. Sevoflurane blocks glioma malignant development by upregulating circRELN through circRELN-mediated miR-1290/RORA axis. BMC Anesthesiol 21:213 (2021). PubMed: 34479497