重组Anti-RON (phospho Y1353)抗体[EPR5598] (ab124671)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5598] to RON (phospho Y1353)
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-RON (phospho Y1353)抗体[EPR5598]
参阅全部 RON 一抗 -
描述
兔单克隆抗体[EPR5598] to RON (phospho Y1353) -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- A431 cell lysates treated with pervanadate; A431 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 9.05 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR5598 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124671于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/50000 - 1/200000. Detects a band of approximately 180 kDa (predicted molecular weight: 152 kDa).
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ICC/IF |
1/500 - 1/1000.
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说明 |
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WB
1/50000 - 1/200000. Detects a band of approximately 180 kDa (predicted molecular weight: 152 kDa). |
ICC/IF
1/500 - 1/1000. |
靶标
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功能
Receptor for macrophage stimulating protein (MSP). Has a tyrosine-protein kinase activity. -
组织特异性
Keratinocytes and lung. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 3 IPT/TIG domains.
Contains 1 protein kinase domain.
Contains 1 Sema domain. -
翻译后修饰
Proteolytic processing yields the two subunits.
Phosphorylated in response to ligand binding. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4486 Human
- Omim: 600168 Human
- SwissProt: Q04912 Human
- Unigene: 517973 Human
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别名
- c met related tyrosine kinase antibody
- CD136 antibody
- CD136 antigen antibody
see all
图片
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All lanes : Anti-RON (phospho Y1353) antibody [EPR5598] (ab124671) at 1/50000 dilution
Lane 1 : A431 cell lysates, untreated
Lane 2 : A431 cell lysates, treated with pervanadate
Predicted band size: 152 kDa -
ab124671, at 1/500 dilution, staining RON in A431 (Human epidermoid carcinoma cell line) cells by Immunofluorescence.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab124671 被引用在 2 文献中.
- Song S et al. EGFR/MET promotes hepatocellular carcinoma metastasis by stabilizing tumor cells and resisting to RTKs inhibitors in circulating tumor microemboli. Cell Death Dis 13:351 (2022). PubMed: 35428350
- de la Fuente C et al. Phosphoproteomic analysis reveals Smad protein family activation following Rift Valley fever virus infection. PLoS One 13:e0191983 (2018). PubMed: 29408900