Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Sample
Human Cell lysate - whole cell (HEK293 and hTERT-RPE1)
Specification
HEK293 and hTERT-RPE1
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in PBS with 0.1% Tween 20
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: ECL donkey anti-rabbit secondary
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Bands
Specific: over 98 and 60 kDa Non-specific: 25, 30, 38, 49, 55 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
Whole cell lysate of HEK293 cells depleted of HOIP.
Positive control
Whole cell lysates of HEK293 cells and hTERT-RPE-1 cells.
Additional data
Additional Notes
Nitrocellulose membrane, iBlot transfer.
Abcam response
Thank you for your review. You may wish to increase the amount of protein you are loading to see good results; we used 15 uf and 50 ug of protein in our testing of Hela cells. We do guarantee this product for human and WB for 12 months from purchase and would be happy to provide a replacement, credit, or refund if your results do not improve.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 31 2014