Application
ChIP
Detection step
Real-time PCR
Sample
Human Cell lysate - nuclear (primary foreskin fibroblasts (infected by human cy)
Specification
primary foreskin fibroblasts (infected by human cy
Negative control
rabbit IgG used as control of phosphor S2 antibody (from the kit (Invitrogen Magnify Chromatin Immunoprecipitation System))
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: 37.5% formaldehyde
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: 37.5% formaldehyde
Positive control
Input sample
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: dilution buffer (from the kit)
Additional data
Additional Notes
1. The experiment was to compare the phosphor S2 ChIP signals of human cytomegalovirus infected cells with or without a specific viral protein (pUL79)
2. ChIP signals of phospho-S2: pUL79-pSer2 and no pUL79-pSer2
ChIP signals of control IgG: pUL79-IgG and no pUL79-IgG
3. The antibody can be used for ChIP assay to detect RNAP II phospho-S2 signal at viral loci (human cytomegalovirus)
4. For ChIP assay using this antibody and magnetic beads like Dynabeads, the nuclear extracts should be "pre-clean" to reduce background. We incubated nuclear extracts with 40ul BSA-preblocked protein A/G Dynabeads (Life Technologies) for 2 hours at 4 degree.
PubMed ID
25166009: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 27 2014