Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (5% gel)
Sample
Schizosaccharomyces pombe Cell lysate - whole cell (whole cell)
Specification
whole cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
1 hour(s) and 0 minute(s)
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit HRP
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: 200 kDa
Exposure
30 second(s)
Additional data
Additional Notes
This antibody works very well for detecting fission yeast RNA polymerase II (Rpb1) in Western blot.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 28 2014