Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Cell lysate - nuclear (human embryonic stem cells)
Specification
human embryonic stem cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS/T + 5% BSA
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Rabbit HRP IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 141 kDa Non-specific: 270 kDa
Exposure
30 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Miguel Fidalgo
Verified customer
提交于 Oct 27 2014