Application
ELISA
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Type
Sandwich (Detection)
Other product details
Incubation time
45 minute(s) · Temperature: 22°C · Diluent: TBS 1X Tween 0.05% milk 5%
Dilution
1/10
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: goat anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 04 2016