Anti-RFX3抗体(ab92670)
Key features and details
- Rabbit polyclonal to RFX3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-RFX3抗体
参阅全部 RFX3 一抗 -
描述
兔多克隆抗体to RFX3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to an internal sequence of Human RFX3.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92670于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 84 kDa.
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IHC-P |
1/50 - 1/100.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 84 kDa. |
IHC-P
1/50 - 1/100. |
靶标
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功能
Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5) (By similarity). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes. Acts as a transcription factor. Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells. -
序列相似性
Belongs to the RFX family.
Contains 1 H-T-H motif winged-type DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5991 Human
- Entrez Gene: 19726 Mouse
- Omim: 601337 Human
- SwissProt: P48380 Human
- SwissProt: P48381 Mouse
- Unigene: 136829 Human
- Unigene: 313321 Mouse
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别名
- bA32F11.1 antibody
- DNA binding protein RFX3 antibody
- Regulatory factor X 3 antibody
see all
图片
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All lanes : Anti-RFX3 antibody (ab92670) at 1/500 dilution
Lane 1 : extracts from RAW264.7 cells
Lane 2 : extracts from COLO cells
Lane 3 : extracts from RAW264.7 cells with immunizing peptide at 5 µg
Lysates/proteins at 10 µg per lane.
Predicted band size: 84 kDa -
Left panel: ab92670 at 1/50 dilution staining RFX3 in paraffin-embedded Human colon carcinoma tissue.
Right panel: same, after pre-incubation with the immunizing peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab92670 被引用在 1 文献中.
- Chen Y et al. Systematic analysis of the Hippo pathway organization and oncogenic alteration in evolution. Sci Rep 10:3173 (2020). PubMed: 32081887