Anti-RFWD3抗体(ab99306)
Key features and details
- Rabbit polyclonal to RFWD3
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RFWD3抗体
参阅全部 RFWD3 一抗 -
描述
兔多克隆抗体to RFWD3 -
宿主
Rabbit -
经测试应用
适用于: IPmore details -
种属反应性
与反应: Human -
免疫原
Immunogen corresponds to a region within amino acids 75-125 of Human RFWD3 (NP_060594.3).
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阳性对照
- Hela whole cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99306于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 RING-type zinc finger.
Contains 3 WD repeats. -
翻译后修饰
Phosphorylated at Ser-46 and Ser-63 upon DNA damage by ATM or ATR. ATM phosphorylation occurs at early times upon DNA damage, while ATR is the major kinase at later times. Phosphorylation by ATM and ATR is required to stabilize p53/TP53. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 55159 Human
- Omim: 614151 Human
- SwissProt: Q6PCD5 Human
- Unigene: 567525 Human
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别名
- BC027246 antibody
- E3 ubiquitin protein ligase RFWD3 antibody
- E3 ubiquitin-protein ligase RFWD3 antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab99306 被引用在 1 文献中.
- Lai Y et al. HERC2 regulates RPA2 by mediating ATR-induced Ser33 phosphorylation and ubiquitin-dependent degradation. Sci Rep 9:14257 (2019). PubMed: 31582797