Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney, Liver, Heart)
Specification
Kidney, Liver, Heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Triton
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1x PBS mit 1,5% serum
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-Rabbit IgG Antibody, (H+L) HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/250
Additional data
Additional Notes
We have tested the RFP-AB on td tomato red in different tissues of a transgenic mouse line.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Stefanie Doppler
Verified customer
提交于 Sep 21 2012