Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (NuPAGE® Novex® 3-8% Tris-Acetate Gel 1.0 mm)
Sample
Human Cell lysate - whole cell (U2OS cells)
Specification
U2OS cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST+1% BSA
Dilution
1/20000
Secondary antibody
Name
Non-Abcam antibody was used: Peroxidase labeled Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 130 kDa
Exposure
5 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Melanie Blasius
Verified customer
提交于 May 20 2013