Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Hippocampal Neurones)
Permeabilization
Yes - 0.1% Triton
Specification
Hippocampal Neurones
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 3% BSA+PBS
Dilution
1/300
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Cy3-AffiniPure Donkey Anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Additional data
Additional Notes
I expect this protein to be localised predominantly in the nuclear structures which it seems to be however, there seems to be unspecific staining around the cytoplasm as well.
Information on the image submitted:
Blue: DAPI, Red: Anti-Red1 and Green:GFP.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 12 2017