重组Anti-RBP4抗体[EP3657] (ab109193)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3657] to RBP4
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-RBP4抗体[EP3657]
参阅全部 RBP4 一抗 -
描述
兔单克隆抗体[EP3657] to RBP4 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human plasma, HepG2 cell lysates, Human fetal kidney, Mouse serum and Rat serum. ICC/IF: HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.18% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP3657 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109193于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
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ICC/IF |
1/100 - 1/250.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
ICC/IF
1/100 - 1/250. |
靶标
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功能
Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. -
疾病相关
Defects in RBP4 are a cause of retinol-binding protein deficiency (RBP deficiency) [MIM:180250]. This condition causes night vision problems. It produces a typical 'fundus xerophthalmicus', featuring a progressed atrophy of the retinal pigment epithelium. -
序列相似性
Belongs to the calycin superfamily. Lipocalin family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 5950 Human
- Entrez Gene: 19662 Mouse
- Entrez Gene: 25703 Rat
- Omim: 180250 Human
- SwissProt: P02753 Human
- SwissProt: Q00724 Mouse
- SwissProt: P04916 Rat
- Unigene: 50223 Human
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别名
- OTTHUMP00000020114 antibody
- OTTHUMP00000020115 antibody
- OTTHUMP00000020116 antibody
see all
图片
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All lanes : Anti-RBP4 antibody [EP3657] (ab109193) at 1/5000 dilution
Lane 1 : HepG2 (human hepatocellular carcinoma) whole cell lysate
Lane 2 : Human fetal kidney
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
Blocking and diluting buffer 5% NFDM/TBST -
Anti-RBP4 antibody [EP3657] (ab109193) at 1/5000 dilution + Mouse serum at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
Blocking and diluting buffer 5% NFDM/TBST -
Anti-RBP4 antibody [EP3657] (ab109193) at 1/5000 dilution + Rat serum at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
Blocking and diluting buffer 5% NFDM/TBST -
All lanes : Anti-RBP4 antibody [EP3657] (ab109193) at 1/1000 dilution (un-purified)
Lane 1 : Human plasma lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa -
Immunocytochemistry/immunofluorescence staining of 100% methanol fixed; 0.1% triton X 100 permeabilized HeLa (human cervix adenocarcinoma) cells with purified ab109193 at dilution of 1/100. The secondary antibody used was Alexa Fluor® 488; goat anti-rabbit IgG (ab150077) at a dilution of 1/1000. Nucleus was counter-stained with DAPI (blue). ab7291, a mouse anti-tubulin antibody (1/1000) was used to stain tubulin along with ab150120 (AlexaFluor®594 goat anti-mouse secondary, 1/1000) shown in the top right hand panel. The negative controls are shown in the bottom middle and right hand panels- for negative control 1 rabbit primary antibody and anti-mouse secondary antibody (ab150120) was used. For negative control 2 mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) was used.
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Immunofluorescent analysis of HeLa cells labelling RBP4 with un-purified ab109193 at a dilution of 1/100.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab109193 被引用在 13 文献中.
- Chen Y et al. A high-resolution route map reveals distinct stages of chondrocyte dedifferentiation for cartilage regeneration. Bone Res 10:38 (2022). PubMed: 35477573
- Li Z et al. miR-24-3p regulation of retinol binding protein 4 in trophoblast biofunction and preeclampsia. Mol Reprod Dev 89:423-430 (2022). PubMed: 35818817
- Saeed A et al. Impaired Hepatic Vitamin A Metabolism in NAFLD Mice Leading to Vitamin A Accumulation in Hepatocytes. Cell Mol Gastroenterol Hepatol 11:309-325.e3 (2021). PubMed: 32698042
- Yoshida Y et al. Alteration of circadian machinery in monocytes underlies chronic kidney disease-associated cardiac inflammation and fibrosis. Nat Commun 12:2783 (2021). PubMed: 33986294
- Welles JE et al. Retinol-binding protein 4 mRNA translation in hepatocytes is enhanced by activation of mTORC1. Am J Physiol Endocrinol Metab 320:E306-E315 (2021). PubMed: 33284085