Anti-RASGRP 4抗体(ab96293)
Key features and details
- Rabbit polyclonal to RASGRP 4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RASGRP 4抗体 -
描述
兔多克隆抗体to RASGRP 4 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to a region within amino acids 108 - 170 (KATGDTQELR RLQICHLVRY WLMRHPEVMH QDPQLEEVIG RFWATVAREG NSAQRRLGDS SDL) of Human RASGRP 4 (NP_733749).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96293于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100.
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WB |
1/500 - 1/3000. Predicted molecular weight: 75 kDa.
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说明 |
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IHC-P
1/100. |
WB
1/500 - 1/3000. Predicted molecular weight: 75 kDa. |
靶标
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功能
Functions as a cation- and diacylglycerol (DAG)-regulated nucleotide exchange factor activating Ras through the exchange of bound GDP for GTP. May function in mast cells differentiation. -
组织特异性
Expressed by mast cells and their progenitors (at protein level). Specifically expressed in mononuclear leukocytes. Highly expressed in myeloid cells compared to lymphoid cells. Also detected in heart, skeletal muscle, spleen, liver, placenta and lung. Not detected in brain. Isoform 1 is the major isoform in normal individuals. Isoform 2 is more significantly expressed in a patient with asthma. Isoform 3 is more significantly expressed in a patient with asthma and a mastocytosis patient. -
序列相似性
Belongs to the RASGRP family.
Contains 1 EF-hand domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-GEF domain. -
发展阶段
Expressed early during development. Detected in all fetal tissues tested. -
结构域
The phorbol-ester/DAG-type zinc finger mediates the binding and the functional activation by DAG. -
细胞定位
Cytoplasm. Cell membrane. Recruited to membranes upon activation by DAG. - Information by UniProt
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数据库链接
- Entrez Gene: 115727 Human
- Omim: 607320 Human
- SwissProt: Q8TDF6 Human
- Unigene: 130434 Human
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别名
- GRP4_HUMAN antibody
- Guanyl nucleotide releasing protein 4 antibody
- RAS guanyl releasing protein 4 antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab96293 被引用在 3 文献中.
- Tang W et al. RasGRP Exacerbates Lipopolysaccharide-Induced Acute Kidney Injury Through Regulation of ERK Activation. Open Forum Infect Dis 9:ofac041 (2022). PubMed: 35198649
- Zhu L et al. The critical role of RasGRP4 in the growth of diffuse large B cell lymphoma. Cell Commun Signal 17:92 (2019). PubMed: 31409422
- Chen X et al. RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma. Cancer Cell 31:685-696.e6 (2017). PubMed: 28486107