重组Anti-RAP1A + RAP1B抗体[EP879Y] (ab75871)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP879Y] to RAP1A + RAP1B
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-RAP1A + RAP1B抗体[EP879Y]
参阅全部 RAP1A + RAP1B 一抗 -
描述
兔单克隆抗体[EP879Y] to RAP1A + RAP1B -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human brain lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP879Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75871于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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说明 |
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WB
1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
靶标
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相关性
RAP1A and RAP1B belong to a family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Human RAP1B is 95% identical to RAP1A. RAP1A and B are proposed to regulate Ras-mediated signalling and may also be involved in the regulation of integrin-mediated cell adhesion, although the mechanism of regulation is not known. -
细胞定位
Cell Membrane; Attached to the membrane by a lipid anchor -
数据库链接
- Entrez Gene: 5906 Human
- Entrez Gene: 5908 Human
- Entrez Gene: 109905 Mouse
- Entrez Gene: 215449 Mouse
- Entrez Gene: 171337 Rat
- Entrez Gene: 295347 Rat
- Omim: 179520 Human
- Omim: RAP1B Human
see all -
别名
- C21KG antibody
- G 22K antibody
- GTP binding protein smg p21A antibody
see all
图片
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Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871) at 1/500 dilution + human brain lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaSecondary antibody - goat anti-rabbit HRP (ab6721)
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Anti-RAP1A + RAP1B antibody [EP879Y] (ab75871) at 1/1000 dilution + Mouse brain
Predicted band size: 21 kDaSecondary antibody - goat anti-rabbit HRP (ab6721)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab75871 被引用在 3 文献中.
- Cho EA et al. Phosphorylation of RIAM by src promotes integrin activation by unmasking the PH domain of RIAM. Structure 29:320-329.e4 (2021). PubMed: 33275877
- Xu B et al. GABAB receptor mediate hippocampal neuroinflammation in adolescent male and female mice after cold expose. Brain Res Bull 142:163-175 (2018). PubMed: 30031816
- Todorovic V et al. Plakophilin 3 mediates Rap1-dependent desmosome assembly and adherens junction maturation. Mol Biol Cell 25:3749-64 (2014). PubMed: 25208567