Application
Western blot
Sample
Human Cell lysate - whole cell (Human gingival epithelial cells)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
20 µg
Specification
Human gingival epithelial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti rabbit HRP
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 42 kDa Non-specific: lower than 37 and 15 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 21 2015