Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116p53wt cells)
Loading amount
30 µg
Specification
HCT116p53wt cells
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey® Blocker 1:1 in PBS + 0.1% Tween
Secondary antibody
Name
Non-Abcam antibody was used: AlexaFluor 680 goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/10000
Detection
Detection method
Odyssey® scanner
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 71 kDa Non-specific: 94 kDa
Positive control
Whole Cell Extract
Negative control
-
Additional data
Additional Notes
Picture legend.
HCT116p53wt Whole Cell Extract.
HCT116p53wt Whole Cell Extract.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 09 2010