Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Primary mouse hippocampal neurons)
Permeabilization
Yes - 0.25 % TX 100
Specification
Primary mouse hippocampal neurons
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1xPBS, 0.1% TX 100, 2%goat serum
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: anti mouse Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
Rab 7is labeled with red fluorescence
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
HERR DR. Vladimir Milenkovic
Verified customer
提交于 Dec 09 2013