Pyruvate dehydrogenase (PDH) WB抗体Cocktail (ab110416)
Key features and details
- Assay type: Quantitative
概述
-
产品名称
Pyruvate dehydrogenase (PDH) WB抗体Cocktail -
检测类型
Quantitative -
种属反应性
与反应: Cow, Human -
产品概述
ab110416 (MSP02) contains a total of 150 µg of monoclonal antibodies reactive against PDH subunits. Isolated mitochondria are available from several species for use as control samples.
The PDH Western blotting kit contains four different mAbs reacting specifically with E1 alpha, E1 beta, E2 and E2/E3bp. A mAb against the OSCP subunit of ATP synthase is included as a control. This mAb mixture can be used to probe the isolated pyruvate dehydrogenase for integrity and purity.
Cocktail Antibodies:
Mouse anti C-V-OSCP [4C11C10D12] monoclonal:
Amount: 12.5 µg
Working concentration: 0.5 µg/mlMouse anti PDH-E1-alpha [9H9AF5] monoclonal:
Amount: 25 µg
Working concentration: 1 µg/mlMouse anti PDH-E1-beta [17A5E2H8] monoclonal:
Amount: 62.5 µg
Working concentration: 2.5 µg/mlMouse anti PDH-E2 [15D3G9C11] monoclonal:
Amount: 25 µg
Working concentration: 1 µg/mlMouse anti PDH-E2/E3bp [13G2AE2BH5] monoclonal:
Amount: 25 µg
Working concentration: 1 µg/ml -
经测试应用
适用于: WBmore details
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 150 µg 150 µg Cocktail of 5 Antibodies 1 x 150µg 1 x 150µg -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110416于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 6 µg/ml. Suggested concentration.
|
说明 |
---|
WB
Use a concentration of 6 µg/ml. Suggested concentration. |
图片
-
Blocked for overnight with 5% Milk/PBS at 4ºC (or 3 hrs at room temperature).
Primary antibody incubatedat 6 µg/mL in 1% milk/PBS for 2hrs at room temperature.
Secondary: HRP-conjugated anti-mouse used according to manufacturers instructions.
Detection method: ECL+
All wash steps carried out in PBS + 0.05% Tween-20.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (30)
ab110416 被引用在 30 文献中.
- Sugiura K et al. MuRF1 deficiency prevents age-related fat weight gain, possibly through accumulation of PDK4 in skeletal muscle mitochondria in older mice. J Orthop Res 40:1026-1038 (2022). PubMed: 34185335
- Hegazy L et al. Identification of Novel Mitochondrial Pyruvate Carrier Inhibitors by Homology Modeling and Pharmacophore-Based Virtual Screening. Biomedicines 10:N/A (2022). PubMed: 35203575
- De Rosa V et al. Non-Canonical Role of PDK1 as a Negative Regulator of Apoptosis through Macromolecular Complexes Assembly at the ER-Mitochondria Interface in Oncogene-Driven NSCLC. Cancers (Basel) 13:N/A (2021). PubMed: 34439291
- Chambers KT et al. Myocardial Lipin 1 knockout in mice approximates cardiac effects of human LPIN1 mutations. JCI Insight 6:N/A (2021). PubMed: 33986192
- Kaya E et al. Beneficial Effects of Acetyl-DL-Leucine (ADLL) in a Mouse Model of Sandhoff Disease. J Clin Med 9:N/A (2020). PubMed: 32276303