Anti-PDHB抗体[17A5E2H8] (ab110331)
Key features and details
- Mouse monoclonal [17A5E2H8] to PDHB
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
概述
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产品名称
Anti-PDHB抗体[17A5E2H8]
参阅全部 PDHB 一抗 -
描述
小鼠单克隆抗体[17A5E2H8] to PDHB -
宿主
Mouse -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human -
免疫原
Full length protein. This information is considered to be commercially sensitive.
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阳性对照
- Isolated mitochondria from Human, Bovine, Rat and Mouse hearts; HepG2 whole cell lysate (ab7900). This antibody gave a positive result in IHC in the following FFPE tissue: Human normal duodenum.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯度
IgG fraction -
纯化说明
ab110331 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
17A5E2H8 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110331于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa.
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说明 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. |
靶标
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功能
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). -
疾病相关
Defects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy). -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 5162 Human
- Entrez Gene: 68263 Mouse
- Entrez Gene: 289950 Rat
- Omim: 179060 Human
- SwissProt: P11966 Cow
- SwissProt: P11177 Human
- SwissProt: Q9D051 Mouse
- SwissProt: P49432 Rat
see all -
别名
- DKFZp564K0164 antibody
- mitochondrial antibody
- ODPB_HUMAN antibody
see all
图片
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IHC image of PDHB staining in Human normal duodenum formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110331, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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All lanes : Anti-PDHB antibody [17A5E2H8] (ab110331) at 1 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 10 µg/ml
Lane 2 : Isolated mitochondria from Bovine heart at 4 µg/ml
Lane 3 : Isolated mitochondria from Rat heart at 10 µg/ml
Lane 4 : Isolated mitochondria from Mouse heart at 10 µg/ml
Lane 5 : HepG2 cell lysate at 20 µg/ml
Predicted band size: 39 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab110331 被引用在 5 文献中.
- Kam JH et al. Mitochondrial absorption of short wavelength light drives primate blue retinal cones into glycolysis which may increase their pace of aging. Vis Neurosci 36:E007 (2019). PubMed: 31199213
- Qi X et al. Knockdown of prohibitin expression promotes glucose metabolism in eutopic endometrial stromal cells from women with endometriosis. Reprod Biomed Online 29:761-70 (2014). PubMed: 25444511
- Halim ND et al. Phosphorylation status of pyruvate dehydrogenase distinguishes metabolic phenotypes of cultured rat brain astrocytes and neurons. Glia 58:1168-76 (2010). PubMed: 20544852
- Han Z et al. A combined therapeutic approach for pyruvate dehydrogenase deficiency using self-complementary adeno-associated virus serotype-specific vectors and dichloroacetate. Mol Genet Metab 93:381-7 (2008). PubMed: 18206410
- Arcelay E et al. Identification of proteins undergoing tyrosine phosphorylation during mouse sperm capacitation. Int J Dev Biol 52:463-72 (2008). PubMed: 18649259