Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Pygopus 2 with ab99274 at 1/200 (1µg/mg). Detection: DAB.
Western blot - Pygopus 2 antibody (ab99274)
All lanes : Anti-Pygopus 2 antibody (ab99274) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH 3T3 whole cell lysate at 50 µg
ab99274, at 0.4 µg/ml, detecting Pygopus 2 in HeLa whole cell lysate by Western blot analysis following Immunoprecipitation (IP). Detection utilized ECL with a 3 second exposure.
For immunoprecipitation, ab99274 was used at 3 µg/mg lysate (1 mg of lysate was used for IP and 20% of IP was loaded).
Lane 1; IP using ab99274
Lane 2; IP using a rabbit anti-Pygopus 2 antibody which recognizes a downstream epitope.
Lane 3; IP using control IgG.