重组Anti-Proteasome subunit alpha type 6抗体[EPR5835] (ab109377)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5835] to Proteasome subunit alpha type 6
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Proteasome subunit alpha type 6抗体[EPR5835]
参阅全部 Proteasome subunit alpha type 6 一抗 -
描述
兔单克隆抗体[EPR5835] to Proteasome subunit alpha type 6 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HepG2, 293T, HeLa and Jurkat cell lysates IHC-P: Human colon tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5835 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109377于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/50000 - 1/200000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
说明 |
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WB
1/50000 - 1/200000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
靶标
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功能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. -
序列相似性
Belongs to the peptidase T1A family. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5687 Human
- Entrez Gene: 26443 Mouse
- Entrez Gene: 29673 Rat
- Omim: 602855 Human
- SwissProt: P60900 Human
- SwissProt: Q9QUM9 Mouse
- SwissProt: P60901 Rat
- Unigene: 446260 Human
see all -
别名
- 27 kDa prosomal protein antibody
- IOTA antibody
- Macropain iota chain antibody
see all
图片
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All lanes : Anti-Proteasome subunit alpha type 6 antibody [EPR5835] (ab109377) at 1/50000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 27 kDa
Observed band size: 27 kDa -
ab109377, at a 1/250 dilution, staining Proteasome 20S alpha 6 in paraffin embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab109377 被引用在 7 文献中.
- Zhang W et al. The potential role of PSMA6 in modulating fat deposition in pigs by promoting preadipocyte proliferation and differentiation. Gene 769:145228 (2021). PubMed: 33096182
- Mishra M et al. Improving the sensitivity of traditional Western blotting via Streptavidin containing Poly-horseradish peroxidase (PolyHRP). Electrophoresis N/A:N/A (2019). PubMed: 31021001
- Sander H et al. Ponceau S waste: Ponceau S staining for total protein normalization. Anal Biochem 575:44-53 (2019). PubMed: 30914243
- Zhu Y et al. Identification of biomarker microRNAs for predicting the response of colorectal cancer to neoadjuvant chemoradiotherapy based on microRNA regulatory network. Oncotarget 8:2233-2248 (2017). WB ; Human . PubMed: 27903980
- Ghosh R et al. Diclofenac induces proteasome and mitochondrial dysfunction in murine cardiomyocytes and hearts. Int J Cardiol 223:923-935 (2016). WB ; Mouse . PubMed: 27589040
- Gilda JE et al. Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS). PLoS One 10:e0135392 (2015). PubMed: 26287535
- Ando R et al. Decreased proteasomal activity causes photoreceptor degeneration in mice. Invest Ophthalmol Vis Sci 55:4682-90 (2014). Mouse . PubMed: 24994871