Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 fibroblasts)
Loading amount
25 µg
Specification
NIH 3T3 fibroblasts
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Milk in PBS with .01% tween 20
Secondary antibody
Name
Non-Abcam antibody was used: mouse anti-goat HRP
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ELC+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 100 kDa Non-specific: None kDa
Additional data
Additional Notes
No complaints here, ab worked as it should for western blot.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 18 2010