Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Cell (cochlea)
Specification
cochlea
Fixative
paraformaldehyde + glutaraldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 22°C
Other product details
Dilution
1/50
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: BBI anti-mouse Fab (GFAF)
Host species: Goat
Clonality: Monoclonal
Conjugation: Gold 10nm
Host species: Goat
Clonality: Monoclonal
Conjugation: Gold 10nm
Dilution
1/50
Additional data
Additional Notes
Postembedding immunogold localization in mouse cochlea produced only low immunogold labeling without a clear pattern. Could be real, but inconclusive.
Antibody was mixed with glycerol 1:1 before dilution.
Antibody was mixed with glycerol 1:1 before dilution.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 30 2011