Application
Western blot
Sample
Cow Tissue lysate - whole (Thymus)
Gel Running Conditions
Reduced Denaturing
Loading amount
45 µg
Treatment
Noninfected controls and virally infected samples
Specification
Thymus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% NFDM
Concentration
0.5 µg/ml
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Donkey Anti-Goat
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Chemiluminescence
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 18 kDa Non-specific: 250, 150, 100, 75, 5 kDa
Additional data
Abcam response
The immunogen of ab9512 has 100% similarity with bovine samples therefore, it is predicted to cross react. In order to obtain positive results, we recommend optimizing the western blot as advised in the link; https://www.abcam.com/protocols/western-blot-troubleshooting-tips. For more information about troubleshooting contact Abcam Scientific Support team.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Katie Knapek
Verified customer
提交于 Apr 18 2018