Application
ChIP
Sample
Human Cell lysate - whole cell (Uterus)
Specification
Uterus
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Semiquantitative PCR
Positive control
RNA Polymerase II
Negative control
Rabbit IgG
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: ChIP Dilution Buffer (Upstate)
Additional data
Additional Notes
We tried a PR antibody from a different company that failed, and we heard that Abcam makes good ChIP antibodies. We bought this one because my boss wanted a monoclonal ab for PR. We took a chance and it worked.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 17 2008