Anti-Presenilin 1/PS-1抗体[APS 18] (ab15458)
Key features and details
- Mouse monoclonal [APS 18] to Presenilin 1/PS-1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-Presenilin 1/PS-1抗体[APS 18]
参阅全部 Presenilin 1/PS-1 一抗 -
描述
小鼠单克隆抗体[APS 18] to Presenilin 1/PS-1 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cynomolgus monkey -
免疫原
Synthetic peptide corresponding to Human Presenilin 1/PS-1 aa 300-400.
Database link: P49768 -
阳性对照
- WB: T-47D, MCF7, Daudi, SH-SY5Y, Caco-2 cell lysate. IHC-P: Human liver and tonsil tissue. ICC: MCF-7 cells, A2058 melanoma cells, mouse fibroblasts and HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
APS 18 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab15458于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500. Predicted molecular weight: 53 kDa.
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IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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说明 |
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WB
1/500. Predicted molecular weight: 53 kDa. |
IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
靶标
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功能
Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Stimulates cell-cell adhesion though its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. May also play a role in hematopoiesis. -
组织特异性
Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes. -
疾病相关
Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3) [MIM:607822]. AD3 is a familial early-onset form of Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
Defects in PSEN1 are a cause of frontotemporal dementia [MIM:600274].
Defects in PSEN1 are the cause of cardiomyopathy dilated type 1U (CMD1U) [MIM:613694]. It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in PSEN1 are the cause of acne inversa familial type 3 (ACNIF3) [MIM:613737]. A chronic relapsing inflammatory disease of the hair follicles characterized by recurrent draining sinuses, painful skin abscesses, and disfiguring scars. Manifestations typically appear after puberty. -
序列相似性
Belongs to the peptidase A22A family. -
结构域
The PAL motif is required for normal active site conformation. -
翻译后修饰
Heterogeneous proteolytic processing generates N-terminal (NTF) and C-terminal (CTF) fragments of approximately 35 and 20 kDa, respectively. During apoptosis, the C-terminal fragment (CTF) is further cleaved by caspase-3 to produce the fragment, PS1-CTF12.
After endoproteolysis, the C-terminal fragment (CTF) is phosphorylated on serine residues by PKA and/or PKC. Phosphorylation on Ser-346 inhibits endoproteolysis. -
细胞定位
Endoplasmic reticulum membrane. Golgi apparatus membrane. Cell surface. Bound to NOTCH1 also at the cell surface. Colocalizes with CDH1/2 at sites of cell-cell contact. Colocalizes with CTNNB1 in the endoplasmic reticulum and the proximity of the plasma membrane. Also present in azurophil granules of neutrophils. - Information by UniProt
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数据库链接
- Entrez Gene: 5663 Human
- Entrez Gene: 19164 Mouse
- Omim: 104311 Human
- SwissProt: P49768 Human
- SwissProt: P49769 Mouse
- Unigene: 3260 Human
- Unigene: 998 Mouse
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别名
- AD3 antibody
- Ad3h antibody
- FAD antibody
see all
图片
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All lanes : Anti-Presenilin 1/PS-1 antibody [APS 18] (ab15458) at 1/500 dilution
Lane 1 : T-47D (human ductal breast epithelial tumor cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate
Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 5 : Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Predicted band size: 53 kDa -
Immunofluorescent analysis of Presenilin 1 / PS-1 using Presenilin 1 / PS-1 Monoclonal antibody (APS 18) ab115458 shows staining in MCF-7 cells. Presenilin 1 / PS-1 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 / PS-1 ab115458 at a dilution of 1:20-1:100 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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Immunofluorescent analysis of Presenilin 1 / PS-1 using Presenilin 1 / PS-1 Monoclonal antibody (APS 18) ab115458 shows staining in A2058 melanoma cells. Presenilin 1 / PS-1 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 / PS-1 ab115458 at a dilution of 1:20-1:100 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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Immunofluorescent analysis of Presenilin 1 / PS-1 using Presenilin 1 / PS-1 Monoclonal antibody (APS 18) ab115458 shows staining in HeLa cells. Presenilin 1 / PS-1 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Presenilin 1 / PS-1 ab115458 at a dilution of 1:20-1:100 over night at 4 ?C washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 / PS-1 ab15458 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 / PS-1 ab15458 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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IF showing PS1 in mouse fibroblasts using ab15458.
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab15458 被引用在 3 文献中.
- Li B et al. ADAM10 mediates ectopic proximal tubule development and renal fibrosis through Notch signalling. J Pathol 252:274-289 (2020). PubMed: 32715474
- Mirzaei M et al. Upregulation of Proteolytic Pathways and Altered Protein Biosynthesis Underlie Retinal Pathology in a Mouse Model of Alzheimer's Disease. Mol Neurobiol N/A:N/A (2019). PubMed: 30707393
- Zoltowska KM et al. Novel interaction between Alzheimer's disease-related protein presenilin 1 and glutamate transporter 1. Sci Rep 8:8718 (2018). PubMed: 29880815