Application
Western blot
Sample
Arabidopsis thaliana Cell lysate - nuclear
Loading amount
5 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
Other product details
Incubation time
2 hour(s) and 0 minute(s)
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Positive control
Recombinant arabidopsis parp (automodified)
Additional data
Additional Notes
Works with Recombinant arabidopsis parp expressed in E coli which is auto PARlylated but not with any of the Arabidopsis samples I used, This does work with a polyclonal from another supplier.
Positive control is also detectable in Westerns, but nothing from plant extracts
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Lee Hunt
Verified customer
提交于 Apr 12 2006