Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
None
Specification
Liver
Blocking step
PBS + 0.1% Triton X-100, 1% BSA, 10% goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS + 0.1% Triton X-100, 1% BSA
Dilution
1/250
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor 488 goat anti-hamster
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Tissues fixed in PBS + 2% formaldehyde for 8 hr at 4C, then cryoprotected in PBS + 30% sucrose overnight at 4C. Stained w/ DAPI.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. justin kenkel
Verified customer
提交于 Jul 28 2012